نوع مقاله : مقاله پژوهشی
عنوان مقاله English
نویسنده English
In this study, the effects of semen diluent solutions on improvement of reproduction performance, by using the criteria such as: the rates of fertilization, percentage of eyed egg and hatching rates were studied. For this purpose eight female broods with average weight and length 1545.62±295.43 g and 54.37±3.85 cm, respectively and sixteen male broods were selected with average weight and length 623.08±180.69 g diluent solutions and 38.91±5.40 cm, respectively. After separation of eggs from coelomic fluids, they were fertilized with sperm using four different diluent solutions: 1- 5.54 g NaCl, 2.422 g Triss, 3.57 g Glycin, regulated in pH=8.4 and solution in 1L distilled water. 2- 7.305 g NaCl, 0.735 g CaCl2.2H2O, regulated in pH=8.4 and solution in 1L distilled water. 3- coelumic fluid that was separated from egg by using net and 4- distilled water as control treatment. The results showed that motility duration of sperm in 1th, 2nd, 3ed and 4th treatments was 65.25±7.36, 30.8±12.83, 73.33±2.117, 40.00±1.61 sec, the duration of forward movement was 6.25±4.71, 15.00±12.63, 52.33±1.90, 34.66±2.51 sec. and percentage eyed eggs was 16.82±6.42, 53.00±4.66, 82.50±3.84, 73.33±2.08, respectively. Percentage hatching was 55.05±5.42, 71.43±0.81, 49.60±6.25, 37.72±4.32, respectively. The results of spermatology parameters, eyed egg percentage and hatching rates showed a significant differences between the 2nd treatment with the rest (P≤0/05). Thus, it can be suggested the 2nd treatment is an effective diluents solution for improving breeding performance of S. zarudnyi.
کلیدواژهها English
