مجله علوم آبزی پروری

مجله علوم آبزی پروری

تأثیر غلظت‌های تحت حاد علف‌کش کلتودیم بر برخی شاخص‌های خونی و بافت‌های سیاه‌ماهی قنات (Capoeta fusca)

نوع مقاله : مقاله پژوهشی

نویسندگان
گروه محیط‌ زیست، دانشکده منابع طبیعی و محیط‌ زیست، دانشگاه بیرجند، بیرجند، ایران.
چکیده
با افزایش جمعیت انسانی و تقاضای محصولات زراعی، استفاده از آفت ­کش­ های طبیعی در دهه اخیر به ­­شدت افزایش یافته است. هدف از این پژوهش تأثیر غلظت ­های تحت حاد علف­ کش کلتودیم بر روی سلول ­های خونی، پارامترهای بیوشیمایی، آنزیم ­های کبدی و آسیب ­های بافت ­شناسی آبشش و روده سیاه ­ماهی قنات می­ باشد. غلظت متوسط کشنده علف­کش کلتودیم در سیاه­ ماهی براساس دستورالعمل OECD محاسبه شد و مطالعات خون ­شناسی و بافت ­شناسی پس از 28 روز بررسی شد. نتایج نشان می­دهد که غلظت متوسط کشنده کلتودیم از 24 ساعت تا 96 ساعت کاهش و سمیت حاد آن افزایش می ­یابد. نتایج نشان می­ دهد که افزایش غلظت علف­ کش کلتودیم سبب کاهش تعداد گلبول‌های قرمز می‌شود. میزان هماتوکریت خون سیاه ­ماهی در معرض گروه کنترل (الف)، 25 (ب)، 50 (ج) و 75 (د) درصد غلظت متوسط کشنده علف­کش کلتودیم پس از 28 روز به‌ترتیب برابر 3/16±28/8، 7/28±28/7، 4/72±28/37و 8/61±23/12می‌باشد بنابراین با افزایش غلظت علف­کش کلتودیم میزان هماتوکریت کاهش یافته است. سطح تری­ گلیسیرید گروه‌های 25 (17/14±125/12)، 50 (14/73±122/82) و 75 (11/23±107/24) درصد نسبت به گروه کنترل (21/17±126/05) کاهش یافته است. سطح آنزیم ­های کبدی در تمامی گروه‌های مورد مطالعه در معرض غلظت‌های مختلف علف­کش کلتودیم به‌طور معنی‌داری (0/05>P) بیشتر از گروه کنترل می‌باشد. آسیب‌های بافت­ شناسی مشاهده شده در بافت آبشش سیاه ­ماهی در معرض علف­کش کلتودیم شامل به هم چسبیدگی و کوتاه شدن لاملاهای ثانویه، اودما و چماقی شدن لاملاها می ­باشد. غلظت­ های تحت حاد علف­کش کلتودیم می ­تواند باعث تغییراتی در سلول­ های خونی و بافت­ های سیاه ­ماهی شود که شدت آن وابسته به غلظت کلتودیم می ­باشد.
کلیدواژه‌ها

عنوان مقاله English

The effect of subacute concentrations of the herbicide clothodim on some blood parameters and tissues of Desert scraper (Capoeta fusca)

نویسندگان English

Zahra Qadri Nookabad
Mohammad Reza Rezaei
Javad Kharkan
Department of Environment, Faculty of Natural Resources and Environment, University of Birjand, Birjand, Iran.
چکیده English

With the increase in human population and demand for crops, the use of natural pesticides has increased dramatically in the last decade. The aim of this study was to investigate the effect of subacute concentrations of the herbicide Clothodim on blood cells, biochemical parameters, liver enzymes, and histological damage to the gills and intestines of Capoeta fusca. The lethal concentration of the herbicide clothodim in blackfish was calculated based on OECD guidelines, and hematological and histological studies were evaluated after 28 days. The results show that the lethal concentration of Clothodim decreases from 24 hours to 96 hours and its acute toxicity increases. The results show that increasing the concentration of the herbicide cloethidium causes a decrease in the number of red blood cells. The hematocrit (%) of the blood of C. fusca exposed to the control group (a), 25 (b), 50 (c) and 75 (d) percent of the lethal concentration of the herbicide clothodim after 28 days was 28.8±3.16, 28.7±7.28, 28.37±4.72 and 23.12±8.61, respectively. Therefore, with increasing concentration of the herbicide Clothodim, the hematocrit level decreased. Triglyceride levels in groups 25 (125.12±17.14), 50 (122.82±14.73) and 75 (107.24±11.23) decreased compared to the control group (126.05±17.21). The levels of liver enzymes in all studied groups exposed to different concentrations of the herbicide clothodim were significantly (P<0.05) higher than the control group. Histological lesions found in the gill tissue of black fish exposed to the herbicide cleothim include adhesion and shortening of secondary lamellae, oedema, and clubbing of lamellae. Subacute concentrations of the herbicide clothodim can cause changes in the blood cells and tissues of C. fusca, the severity of which depends on the concentration of clothodim.  

کلیدواژه‌ها English

Aqueduct
Biochemical Parameters
Histology
Pesticide
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