مجله علوم آبزی پروری

مجله علوم آبزی پروری

تأثیر عصاره خشک خرفه (Portulaca oleracea) جیره بر روی فعالیت آنزیم‌های کبدی و مقادیر اسیدهای چرب فیله بچه‌ماهی قزل‌آلای رنگین‌کمان (Oncorhynchus mykiss)

نوع مقاله : مقاله پژوهشی

نویسندگان
مرکز تحقیقات ماهیان سردآبی، مؤسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تنکابن، ایران.
چکیده
در این مطالعه، تأثیر استفاده از عصاره خشک گیاه خرفه (Portulaca oleracea) جیره بر روی فعالیت آنزیم­ های کبدی سرم و مقادیر اسیدهای چرب فیله بچه‌ماهی قزل ­آلای رنگین­ کمان (Oncorhynchus mykiss) مورد بررسی قرار گرفت. جیره ­ها در 5 سطح صفر (T0)، 0/5 درصد (T1)، 1 درصد (T2)، 5/1 درصد (T3) و 2 درصد (T4) از پودر عصاره خشک خرفه برای سه تکرار تهیه شدند. ماهیان با متوسط وزن اولیه 0/1±9/37 گرم به میزان سه درصد وزن بدن و 4 وعده در روز با وعده ­های یکسان از ساعت 8 صبح تا 4 بعدازظهر به‌مدت 56 روز غذادهی شدند. در پایان 8 هفته آزمایش، فعالیت آنزیم ­های کبدی آلانین آمینوترانسفراز، آسپارتات آمینوترانسفراز و آلکالین فسفاتاز سرم و مقادیر اسیدهای چرب فیله بچه‌ماهی قزل­ آلای رنگین ­کمان اندازه­ گیری شد. براساس نتایج به‌دست آمده، بالاترین مقادیر آنزیم ­های آلانین آمینوترانسفراز، آسپارتات آمینوترانسفراز و آلکالین فسفاتاز در سرم ماهی قزل‌آلای رنگین­ کمان در سطوح 1–0/5 درصد گیاه خرفه جیره به‌دست آمد (0.05>P). این نشان‌دهنده اثر منفی عصاره خرفه بر روی آنزیم‌های کبدی سرم بچه‌ماهی قزل­ آلای رنگین­ کمان خصوصاً در مقادیر 1–0/5 درصد عصاره گیاهی در جیره بود. مقادیر کل اسیدهای چرب اشباع (SFA) در فیله ماهی با افزایش سطح گیاه خرفه نسبت به تیمار شاهد کاهش معنی­ داری نشان داد، به‌طوری‌که کمترین مقدار آن در سطح 2 درصد عصاره گیاهی به‌دست آمد (0.05>P). به ­طور معکوس، مقادیر کل اسیدهای چرب تک غیراشباع (MUFA) در فیله ماهی با افزایش سطح گیاه خرفه نسبت به تیمار شاهد افزایش یافت (0.05>P)، به­ طوری­ که بیشترین مقدار آن در سطح 1.5 درصد عصاره گیاهی به‌دست آمد. باجود اینکه در سطوح بالاتر از 1 درصد گیاه خرفه جیره، مقدار کل اسیدهای چرب چندغیراشباع (PUFA) در فیله ماهی نسبت به تیمار شاهد کاهش معنی­دار نشان داد (0.05>P) نسبت اسیدهای چرب چندغیراشباع n-6/n-3 در فیله ماهی افزایش یافت که بیانگر تأثیر مثبت استفاده از گیاه خرفه بر روی کیفیت فیله بچه ماهی قزل ­آلای رنگین ­کمان بود. همچنین، این اثرگذاری وابسته به مقدار گیاه در جیره ماهی بود، به‌طوری­ که بیشترین تأثیر عصاره گیاهی بر این نسبت در فیله ماهی در سطح 2-1/5 درصد جیره به‌دست آمد.
کلیدواژه‌ها

عنوان مقاله English

The effect of dietary dried purslane (Portulaca oleracea) extract on the activity of liver enzymes and fatty acid levels of rainbow trout (Oncorhynchus mykiss) juvenile fillet

نویسندگان English

Masoud Kousha
Mehdi Mohammad Alikhani
Saltanat Najjar Lashgari
Coldwater Fishes Research Center, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization, Tonekabon, Iran.
چکیده English

This study investigated the effect of dietary dried purslane (Portulaca oleracea) extract on the activity of serum liver enzymes and fatty acid levels of rainbow trout (Oncorhynchus mykiss) juvenile fillet. The diets were prepared at five levels of zero (T0), 0.5% (T1), 1% (T2), 1.5% (T3), and 2% (T4) of purslane extract for three replicates. The fish with an average initial weight of 9.0±1.37 g was fed four times in equal meals from 8:00 to 16:00 for 56 days. At the end of the 8-week experiment, the activities of liver enzymes, including alanine aminotransferase, aspartate aminotransferase, and serum alkaline phosphatase, and fatty acids levels of rainbow trout juveniles’ fillet were measured. According to the obtained results, the highest levels of alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase enzymes in rainbow trout (O. mykiss) serum were obtained at 0.5-1% of dietary purslane (P <0.05). These results indicated a negative effect of purslane (P. oleracea) extract on the activity of liver enzymes of rainbow trout juveniles, especially at 0.5-1% dietary plant extract. The total saturated fatty acid (SFA) levels in fish fillets decreased significantly with increasing levels of dietary purslane compared to the control treatment. Therefore, the lowest levels were obtained at 2% dietary plant extract (P<0.05). Conversely, the total amount of monounsaturated fatty acids (MUFA) in fish fillet increased with increasing levels of dietary purslane compared to the control treatment (P<0.05), in which the highest amount was acquired at 1.5% dietary plant extract. Although the total amount of polyunsaturated fatty acids (PUFA) in fish fillet was decreased significantly compared to the control treatment (P<0.05) at levels higher than 1% of dietary purslane, the ratio of n-3/n-6 PUFAs in fish fillet increased. Consequently, this indicated a positive effect of purslane extract on the quality of rainbow trout juvenile fillet. Also, this effect depended on the amount of plant extract in the fish diet. The most significant effect of plant extract on this ratio in fish fillets was obtained at 1.5-2% of the diet. Therefore, using purslane extract at 1.5-2% of the diet, despite the negative effect on the activity of some serum liver enzymes, improved the quality of rainbow trout juvenile fillet.

کلیدواژه‌ها English

Medicinal plants
rainbow trout
Liver enzymes
Fatty acids
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