مجله علوم آبزی پروری

مجله علوم آبزی پروری

اثرات فـرم‌های آلی، معدنی و نانـوذرات عناصـر روی و منگنز بر رشد، تکـوین و بازماندگی لارو مـاهی شانک زردباله (Acanthopagrus arabicus) با استفاده از روش غنی‌سـازی غذای زنده

نوع مقاله : مقاله پژوهشی

نویسندگان
1 پژوهشکده آرتمیا و آبزی‌پروری دانشگاه، ارومیه، ارومیه، ایران.
2 پژوهشکده آبزی‌پروری جنوب کشور، اهواز، ایران.
3 پژوهشکده خلیج فارس، دانشگاه خلیج فارس، بوشهر، ایران.
4 انستیتو ایرتا، واحد آبزی‌پروری، اسپانیا.
چکیده
جهت بررسی اثرات غنی‌سازی غذاهای زنده با منابع مختلف روی (Zn) و منگنز (Mn) بر لارو ماهی شانک زردباله، یک دوره پرورشی به‌مدت 42 روز انجام پذیرفت. چهار منبع مختلف Zn و Mn برای غنی‌سازی روتیفر و آرتمیا استفاده شد: جلبک غنی‌شده با سولفات Zn-Mn، نانوذرات  ZnوMn ، فرم آلی (گلیسینات  ZnوMn) و فرم معدنی (سولفات Zn وMn ). گروه شاهد بدون غنی‌سازی بود. لاروها در مخازن استوانه‌ای حاوی آب دریا توزیع شدند و با روتیفر و آرتمیا تغذیه شدند. در پایان دوره، مقاومت ماهیان در برابر تنش‌های محیطی مورد سنجش قرار گرفت. نتایج به‌دست آمده از بررسی نمونه‌ها در پایان دوره نشان داد، لاروهایی که با جلبک غنی‌شده با سولفاتZn-Mn  و غذاهای زنده غنی‌شده با Zn-Mn آلی تغذیه شده بودند، وزن مرطوب و طول کل بیشتری داشتند. گروه شاهد، جلبک غنی‌شده با Zn-Mn و غذاهای زنده غنی‌شده با نانو ذرات Zn-Mn، فعالیت کاتالاز بیشتری داشتند. همچنین لاروهایی که با غذاهای زنده غنی‌شده با Zn-Mn تغذیه شدند، فعالیت‌های آنتی‌اکسیدانی بیشتری نسبت به گروه شاهد نشان دادند. میزان فعالیت آنزیم آمیلاز در گروه شاهد در مقایسه با سایر تیمارها پایین‌تر بود؛ اگرچه این اختلاف با تیمار آلی معنی‌دار نبود. فعالیت آنزیم کیموتریپسین در گروه شاهد به‌طور معنی‌داری کمتر از سایر تیمارها بود؛ همچنین فعالیت آنزیم تریپسین در گروه شاهد کمتر بود درحالی‌که این شاخص در تیمارهای غنی شده با مواد نانو و معدنی به‌طوری معنی‌دار بیشتر از سایر تیمارها بود. غنی‌سازی غذای زنده با Zn وMn  از طریق غنی‌سازی جلبک با منابع آلی می‌تواند منجر به بهبود عملکرد متابولیکی و عملکرد رشد بالاتر، کاهش استرس اکسیداتیو و افزایش ظرفیت آنتی‌اکسیدانی و نیز تقویت مکانیسم‌های دفاع سلولی در لاروهای Acanthopagrus arabicus شود.
کلیدواژه‌ها

عنوان مقاله English

The effects of organic, inorganic and nanoparticle forms of zinc and manganese on the growth, development and survival of larvae in the yellowfin tuna (Acanthopagrus arabicus) using the live food enrichment method

نویسندگان English

Reza Farshadian 1
Farzaneh Noori 1
Mansour Torfi Mozanzadeh 2
Seyed Ahmad Ghasemi 3
Enric Gisbert 4
1 Artemia and Aquaculture Research Institute, Urmia University, Urmia, Iran.
2 South Iran Aquaculture Research Institute, Ahvaz, Iran.
3 Persian Gulf Institute, Persian Gulf University, Bushehr, Iran.
4 IRTA, Aquaculture Program, La Ràpita, Spain.
چکیده English

To investigate the effects of live food enrichments with various sources of zinc (Zn) and manganese (Mn) on yellowfin seabream larvae (Acanthopagrus arabicus), a 42-day rearing period was conducted. Four different Zn and Mn sources were used to enrich rotifers and Artemia: enriched algae with Zn-Mn sulphate, nanoparticles of Zn-Mn, organic Zn-Mn glycinate, and inorganic Zn-Mn sulphate. The control group was left unenriched. The larvae were distributed in cylindrical tanks containing seawater and fed with enriched rotifers and Artemia. At the end of the rearing period, the resistance of fish to environmental stress was evaluated. The results obtained from samples collected at the end of the period revealed that larvae fed with algae enriched with Zn-Mn and live food enriched with organic Zn-Mn demonstrated higher wet weight and total length. The control group, algae enriched with Zn-Mn, and live food enriched with Zn-Mn nanoparticles showed increased catalase activity. Larvae fed enriched live food exhibited higher antioxidant activities compared to the control group. The amylase enzyme activity was lower in the control group than in other treatments, although this difference was insignificant compared to the organic treatment. The chymotrypsin and trypsin enzyme activity in the control group was significantly lower than in other treatments. However, this indicator was significantly higher in treatments fed diets enriched with nano and inorganic Zn-Mn compared to other treatments. The live food enriched with Zn and Mn through algae enrichment using organic sources can lead to improved metabolic performance, enhanced growth rates, reduced oxidative stress, increased antioxidant capacity, and strengthened cellular defense mechanisms in the larvae of Acanthopagrus arabicus.

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