مجله علوم آبزی پروری

مجله علوم آبزی پروری

ارزیابی بیوانفورماتیک نقش MicroRNAها در تنظیم بیان ژن‌های دخیل در سیستم ایمنی ماهی گورخری ( Danio rerio)

نوع مقاله : مقاله پژوهشی

نویسندگان
مهدی بنایی 1
رضا شاکری 2
1 گروه شیلات، دانشکده منابع طبیعی، دانشگاه صنعتی خاتم‌الانبیا بهبهان، بهبهان، ایران
2 گروه محیط‌زیست، دانشکده منابع طبیعی، دانشگاه صنعتی خاتم‌الانبیا بهبهان، بهبهان، ایران.
چکیده
این مطالعه به بررسی نقش microRNAها در تنظیم بیان ژن‌های دخیل در سیستم ایمنی ماهی گورخری از طریق تحلیل بیوانفورماتیکی پرداخته است. بدین‌منظور، پروفایل­های ژنی مرتبط با سیستم ایمنی ماهیان استخوانی از پایگاه داده NCBI استخراج و microRNAهای هدف ­گیرنده این ژن‌ها با استفاده از ابزار Target Scan و miRmap شناسایی شدند. نتایج نشان داد که ژن‌هایی نظیر tlr3، nlrx1، saa،cxcl12a ، C3، mhc1ze و  mhc2dabنقش مهمی در پاسخ‌های ایمنی، التهاب و استرس اکسیداتیو ایفا می­کنند. از میان آنها، microRNAهای dre-miR-30e-3p و dre-miR-723-5p با امتیازهای بالاتر از 80 به‌طور مؤثری ژن tlr3 را که در تشخیص RNA دو رشته‌ای و فعال‌سازی پاسخ‌های ایمنی ذاتی نقش دارند، تنظیم می­ کنند. همچنین، dre-miR-155 و dre-miR-2194 و dre-miR-2185-5p به‌عنوان تنظیم‌کننده‌های اصلی در تنظیم ژن nlrx1، که نقش مهمی در مسیرهای ایمنی و استرس اکسیداتیو دارد، شناخته شدند. بیان ژنsaa ، دخیل در پاسخ‌های التهابی و تنظیم ایمنی، به‌وسیلة dre-miR-135c،dre-miR-135b  و dre-miR-135a تنظیم می ­شود. همچنین، ژن cxcl12a که کموکین SDF-1 را رمزگذاری می‌کند و در مهاجرت سلول‌های ایمنی نقش دارد، توسط dre-miR-740، dre-miR-22b و dre-miR-22a تنظیم می‌شود. از سوی دیگر، ژن‌های سیستم مکمل مانند C3 تحت تأثیر dre-miR-93 و dre-miR-128 قرار دارند. ژن‌های mhc1ze وmhc2dab، microRNAهایی نظیر dre-miR-101a، dre-miR-3906 و dre-miR-740 به‌عنوان تنظیم‌کننده‌های قوی شناسایی شدند. این نتایج، همراه با تحلیل‌های دقیق‌تری که از ترکیب داده‌های بیوانفورماتیک حاصل شده است، به شناسایی و درک نقشmicroRNAها در تنظیم سیستم ایمنی ماهی گورخری کمک کرده و به‌عنوان مبنای مطالعات تجربی آینده در این حوزه قرار می‌گیرند.
کلیدواژه‌ها
microRNAها
سیستم ایمنی
تنظیم بیان ژن
آنالیز تعاملاتmRNA-miRNA
ماهی گورخری

عنوان مقاله English

Bioinformatics assessment of the role of MicroRNAs in regulating the expression of genes involved in the immune system of zebrafish (Danio rerio)

نویسندگان English

Mahdi Banaee 1
Reza Shakeri 2
1 Department of Aquaculture, Faculty of Natural Resources, Behbahan Khatam Alanbia University of Technology, Behbahan, Iran.
2 Department of Environment, Faculty of Natural Resources, Behbahan Khatam Alanbia University of Technology, Behbahan, Iran.
چکیده English

This study investigated the role of microRNAs in regulating the expression of genes involved in the immune system of zebrafish through bioinformatics analysis. To achieve this, gene profiles related to the immune system of teleost fish were extracted from the NCBI website, and microRNAs targeting these genes were identified using the TargetScan and miRmap tools. The results revealed that genes such as tlr3, nlrx1, saa, cxcl12a, C3, mhc1ze, and mhc2dab play a crucial role in immune responses, inflammation, and oxidative stress. Notably, microRNAs dre-miR-30e-3p and dre-miR-723-5p, with scores exceeding 80, effectively regulated the tlr3 gene, which is essential for recognizing double-stranded RNA and activating innate immune responses. Additionally, dre-miR-155, dre-miR-2194, and dre-miR-2185-5p, were identified as key regulators of the nlrx1 gene, which is pivotal in immune pathways and oxidative stress. The expression of the saa gene, which is involved in inflammatory responses and immune regulation, is regulated by dre-miR-135c, dre-miR-135b, and dre-miR-135a. Similarly, the cxcl12a gene, which encodes the chemokine SDF-1 and plays a role in immune cell migration, is regulated by dre-miR-740, dre-miR-22b, and dre-miR-22a. Furthermore, complement system genes such as C3 are influenced by dre-miR-93 and dre-miR-128. The major histocompatibility complex genes mhc1ze and mhc2dab are strongly regulated by microRNAs such as dre-miR-101a, dre-miR-3906, and dre-miR-740. These findings, supported by comprehensive bioinformatics analyses, provide valuable insights into the role of microRNAs in regulating the zebrafish immune system and serve as a foundation for future experimental studies in this field.

کلیدواژه‌ها English

microRNAs
Immune system
Gene expression regulation
mRNA-miRNA interaction analysis
Zebrafish
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مجله علوم آبزی پروری
دوره 12، شماره 2
آذر 1403
صفحه 91-105