مجله علوم آبزی پروری

مجله علوم آبزی پروری

بررسی اثر درمانی مکمل خوراکی و ضماد جلبک Spirulina platensisبر شاخص‌های بیوشیمیایی و ایمنی در ماهی قزل‌آلای رنگین‌کمان (Walbaum, 1792) Oncorhynchus mykiss پس از ایجاد جراحت جلدی

نوع مقاله : مقاله پژوهشی

نویسندگان
1 گروه شیلات، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران.
2 مرکز تحقیقات سلول های بنیادی و بخش فارماکولوژی دانشکده پزشکی، دانشگاه علوم پزشکی شیراز، ایران.
3 گروه شیلات، دانشکده منابع طبیعی دانشگاه صنعتی خاتم الانبیا (ص) بهبهان، ایران.
چکیده
پوست به‌عنوان اولین سد محافظ در برابر صدمات فیزیکی و عوامل بیماری‌زا عمل می‌کند. ریزجلبک‌های اسپیرولینا به‌دلیل داشتن ریزمغذی ­هایی مانند اسیدهای چرب ضروری، اسیدهای آمینه ضروری و غیره می­ تواند در بهبود آسیب‌های پوستی ماهیان، مورد استفاده قرار گیرد. در این تحقیق، از 360 قطعه ماهی قزل‌آلای رنگین‌کمان با وزن 50 گرم (با اخذ کد اخلاق به شماره 9821316001) در دانشگاه علوم کشاورزی و منابع طبیعی گرگان استفاده شد. قبل از انجام آزمایش نهایی، تأثیر غلظت ­های 1 تا 15 درصد عصاره اسپیرولینا بر ماهیان مورد ارزیابی قرار گرفت. سپس سه دوز 3، 7 و 10 درصد عصاره اسپیرولینا جهت آزمایش به‌صورت خوراکی و ضماد انتخاب گردید. پس از ایجاد جراحت جلدی ماهیان به‌مدت 28 روز تحت درمان به شیوه خوراکی و استفاده از ضماد قرار گرفتند. در روز 28، از ماهیان خون­گیری شد و پارامترهای بیوشیمیایی و ایمنی اندازه ­گیری گردید. افزایش سطح گلوکز، کلسترول، تری‌گلیسیرید، پروتئین تام، گلوبولین و ایمونوگلوبولین پلاسما و افزایش فعالیت لیزوزیم پس از آسیب پوستی بیانگر پاسخ­ های فیزیولوژیکی برای بهبودی و بازگرداندن یکپارچگی بافت است. نتایج این مطالعه نشان داد که تجویز مکمل‌های اسپیرولینا به‌خصوص گروه خوراکی با غلظت 7 درصد که از پماد با همین غلظت استفاده کرده بودند اثرات مفیدی بر پارامترهای فیزیولوژیکی مختلف در ماهی‌های آسیب دیده دارند.
کلیدواژه‌ها

عنوان مقاله English

Investigating the therapeutic effect of Spirulina platensis algae supplement and poultice on biochemical parameters and immune parameters in rainbow trout Oncorhynchus mykiss (Walbaum, 1792) after skin injury

نویسندگان English

Mohammad Saeid Fereidouni 1
Rasuol Ghorbani 1
Nader Tanideh 2
Abdul Majid Haji-Moradlou 1
Seyed Abbas Hosseini 1
Mahdi Banaee 3
Hadithseh Kashiri 1
1 Department of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Iran.
2 Stem cell research center and pharmacology department, Faculty of Medicine, Shiraz University of Medical Sciences, Iran.
3 Department of Aquaculture, Faculty of Natural Resources, Khatam Alanbia University of Technology, Behbehan, Iran.
چکیده English

The skin is the first protective barrier against physical injuries and pathogens. Spirulina microalgae can be used to improve skin damage in fish due to its content of micronutrients, such as essential fatty acids, essential amino acids, and more. In this study, 360 rainbow trout weighing 50 grams each (with ethical code number 9821316001) were used at Gorgan University of Agricultural Sciences and Natural Resources. Before the final test, the effects of spirulina extract at concentrations ranging from 1% to 15% were evaluated on the fish. Subsequently, three doses—3%, 7%, and 10%—were selected for oral and poultice testing. After inducing skin injuries, the fish were treated both orally and with poultices for 28 days. On the 28th day, blood samples were collected, and biochemical and immune parameters were measured. The observed increases in glucose, cholesterol, triglycerides, total protein, globulin, and immunoglobulin plasma levels, along with elevated lysozyme activity after skin damage, indicate physiological responses aimed at recovery and the restoration of tissue integrity. The results of this study showed that administering spirulina supplements, particularly in the group that received a 7% concentration orally and used an ointment of the same concentration, had beneficial effects on various physiological parameters in injured fish.

کلیدواژه‌ها English

Wound
Spirulina
rainbow trout
Biochemical
Immunity
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