بررسی آسیب‌های بافت‌شناسی فلزات سنگین مختلف بر روی ماهی قنات (Capoeta fusca)

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه محیط زیست، دانشکده منابع طبیعی و محیط زیست، دانشگاه بیرجند، بیرجند، ایران.

چکیده

یکی از مهم­ترین آلاینده ­های منابع آب، فلزات ­سنگین می ­باشد. توسعه فعالیت­ های صنعتی و کشاورزی منجر به توزیع گسترده آن­ ها در محیط شده و نگرانی‌هایی را در مورد اثرات بالقوه آن‌ها بر اکوسیستم و سلامت انسان ایجاد کرده است. این پژوهش با هدف بررسی آسیب­ های بافت­ شناسی فلزات ­سنگین کروم، کادمیوم، کبالت و مس بر روی ماهی قنات می­ باشد. در این پژوهش سیاه ­ماهی (Capoeta fusca) در 5 گروه 10 قطعه ­ای (یک گروه کنترل) در معرض غلظت زیرکشندگی کلریدهای کروم، کادمیوم، کبالت و مس  به مدت 28 روز قرار گرفتند و پس از پایان 28 روز سه قطعه ماهی به‌صورت تصادفی از هر گروه تیمار برداشته و تشریح شدند. آسیب­ های بافت­ شناسی در آبشش ماهیان در معرض کلرید کبالت شامل اودما، همجوشی و کوتاه شدن لاملاهای ثانویه می ­باشد در بافت روده برخی از آسیب­ های بافت ­شناسی به­‌دلیل قرار گرفتن در معرض کلرید­های کروم، کادمیوم، کبالت و مس شامل تورم سلول­ های گابلت، افزایش تعداد سلول ­های گابلت، تخریب ویلی و گسترش ساختار ویلی می­ باشد. شدت  آسیب­ های بافت­ شناسی در روده ماهیان در معرض کلرید مس بیشتر از ماهیان در معرض کلرید­های کروم، کادمیوم و کبالت می­ باشد. غلظت ­های زیرکشندگی فلزات ­سنگین مختلف می ­تواند اثرات نامطلوبی بر بافت آبشش و روده سیاه ­ماهی داشته باشد که شدت آن با گذشت زمان افزایش می ­یابد.

کلیدواژه‌ها


عنوان مقاله [English]

Investigating the histological damage of different heavy metals on aqueduct fish (Capoeta fusca)

نویسندگان [English]

  • Mohammad Hossein Sayadi
  • Javad Kharkan
Department of Environmental Engineering, Faculty of Natural Resources and Environment University of Birjand, Birjand, Iran.
چکیده [English]

One of the most important pollutants in water sources is heavy metals. The development of industrial and agricultural activities has led to their widespread distribution in the environment and has raised concerns about their potential effects on the ecosystem and human health. This research aims to investigate the histological damage of heavy metals chromium, cadmium, cobalt, and copper on aqueduct fish. In this study, Capoeta fusca in 5 groups each with 10 specimens (one control group) were exposed to sublethal concentrations of Cr, Cd, Co, and Cu chlorides. They were exposed to these heavy metals for 28 days. The histological damage in the gills of fishes exposed to cobalt chloride includes edema, a fusion of lamellae and lamellar synechiae, and in the gills of fish exposed to copper chloride, it includes necrosis, the fusion of lamellae and lamellar synechiae. In the intestinal tissue, some histological damages due to exposure to chromium, cadmium, cobalt and copper chlorides include the swelling of goblet cells, increase in the number of goblet cells, destruction of villi and expansion of villi structure. The severity of histological damage in the intestine of fish exposed to copper chloride is higher than that of fish exposed to chromium, cadmium and cobalt chlorides. Sub-lethal concentrations of various heavy metals can have adverse effects on the gill tissue and intestine of C. fusca, the severity of which increases with time.

کلیدواژه‌ها [English]

  • Environmental pollution
  • Heavy metals
  • Fusion of lamellae
  • Goblet cells
هدایتی ع. ا. 1393. سم شناسی آبزیان. انتشارات دانشگاه علوم کشاورزی و منابع طبیعی گرگان. گرگان. چاپ اول، 212 صفحه.
Abel P., Axiak V. 1990. Ecotoxicology and the marine environment, Old University Building. Malta, 250-269.
Bais U.E., Lokhande M.V. 2012. Effect of cadmium chloride on histopathological changes in the freshwater fish Ophiocephalus striatus (Channa). International Journal of Zoological Research 8(1), 23.‏
Barišić J., Marijić V.F., Mijošek T., Čož-Rakovac R., Dragun Z., Krasnići N., Erk M. 2018. Evaluation of architectural and histopathological biomarkers in the intestine of brown trout (Salmo trutta Linnaeus, 1758) challenged with environmental pollution. Science of the Total Environment 642, 656-664.
Batool M., Abdullah S., Ijaz M.U., Kousar S., Fatima M., Ilyas R., Mughal K.T. 2018. Heavy Metals (Cadmium and Lead) Induced Oxidative Stress in Channa marulius and Wallago Attu during Acute Toxicity Experiments. Pakistan Journal of Zoology Supplementary Series 13, 74-79.‏‏
Beegam A., Lopes M., Fernandes T., Jose J., Barreto A., Oliveira M., Pereira M.L. 2020. Multiorgan histopathological changes in the juvenile seabream Sparus aurata as a biomarker for zinc oxide particles toxicity. Environmental Science and Pollution Research 27(25), 30907-30917.‏
Bonsignore M., Manta D.S., Mirto S., Quinci E.M., Ape F., Montalto V.,  Sprovieri M. 2018. Bioaccumulation of heavy metals in fish, crustaceans, molluscs and echinoderms from the Tuscany coast. Ecotoxicology and Environmental Safety 162, 554-562.
Bortey-Sam N., Nakayama S.M., Ikenaka Y., Akoto O., Baidoo E., Yohannes Y.B., Ishizuka M. 2015. Human health risks from metals and metalloid via consumption of food animals near gold mines in Tarkwa, Ghana: Estimation of the daily intakes and target hazard quotients (THQs). Ecotoxicology and Environmental Safety 111, 160-167.
Capaldo A., Gay F., Laforgia V. 2019. Changes in the gills of the European eel (Anguilla anguilla) after chronic exposure to environmental cocaine concentration. Ecotoxicology and Environmental Safety 169, 112-119.‏‏
Choudhary L., Vyas T., Chauhan N.R.S., Madhavi B., Yadav G.K., Bharadwaj S. 2019. Histopathological changes due to lead toxicity in gills of P. ticto (hem). International Research Journal of Science and Engineering 7(4), 92-95.
Eagderi S., Mouludi-Saleh A., Esmaeli H.R., Sayyadzadeh G., Nasri M. 2022. Freshwater lamprey and fishes of Iran; a revised and updated annotated checklist-2022. Turkish Journal of Zoology 46(6), 500-522.
Elahee K.B., Bhagwant S. 2007. Hematological and gill histopathological parameters of three tropical fish species from a polluted lagoon on the west coast of Mauritius. Ecotoxicology and Environmental Safety 68(3), 361-371.‏
Fahmi U., Andriani I., Salmah S., Hatta T.H., Omar S.B.A., Sari D.K. 2019. Histopathology of liver and intestine of pangkilan bare fish (Oryzias matanensis) Polluted by nickel and iron in Lake Matano, South Sulawesi. In IOP Conference Series: Earth and Environmental Science 370(1), 012078.
FAO/WHO 2016. Accumulation of heavy metals in fishes of freshwater. Available from: https://www.slideshare.net. Accessed on 3 August
2021.
Farag A.M., Nimick D.A., Kimball B.A., Church S.E., Harper D.D., Brumbaugh W.G. 2007. Concentrations of metals in water, sediment, biofilm, benthic macroinvertebrates, and fish in the Boulder River watershed, Montana, and the role of colloids in metal uptake. Archives of Environmental Contamination and Toxicology 52(3), 397-409.‏
Fonseca A.R., Fernandes L.S., Fontainhas-Fernandes A., Monteiro S.M., Pacheco F.A.L. 2016. From catchment to fish: Impact of anthropogenic pressures on gill histopathology. Science of the Total Environment 550, 972-986.‏
Gawad S.S.A. 2018. Acute toxicity of some heavy metals to the fresh water snail, Theodoxus niloticus (Reeve, 1856). The Egyptian Journal of Aquatic Research 44(2), 83-87.‏
Genchi G., Carocci A., Lauria G., Sinicropi M.S., Catalano A. 2020. Nickel: Human health and environmental toxicology. International Journal of Environmental Research and Public Health 17(3), 679.‏
Gernhöfer M., Pawert M., Schramm M., Müller E., Triebskorn R. 2001. Ultrastructural biomarkers as tools to characterize the health status of fish in contaminated streams. Journal of Aquatic Ecosystem Stress and Recovery 8(3), 241-260.‏
Hamelink J., Landrum P.F., Bergman H., Benson W.H. 1994. Bioavailability: physical, chemical, and biological interactions. CRC press.
Hussan A., Choudhury T.G., Ahmed I., Gita S., Das A., Udit U.K., Mandal R.N. 2018. Effect of mercury and cadmium on the oxygen consumption and gill Histology of Catla catla (Ham. 1822). Proceedings of the National Academy of Sciences, India Section B: Biological Sciences 88(2), 729-738.‏‏
Jalaludeen M.D., Arunachalam M., Raja M., Nandagopal S., Showket A.B., Sundar S., Palanimuthu D. 2012. Histopathology of the gill, liver and kidney tissues of the freshwater fish Tilapia mossambica exposed to cadmium sulphate. International Journal of Advanced Biological Research 2(4), 572-578.‏
Jamroz D., Wertelecki T., Houszka M., Kamel C. 2006. Influence of diet type on the inclusion of plant origin active substances on morphological and histochemical characteristics of the stomach and jejunum walls in chicken. Journal of Animal Physiology and Animal Nutrition 90(5‐6), 255-268.‏
Johari S.A., Coad B.W., Mazloomi S., Kheyri M., Asghari S. 2009. Biological and morphometric characteristics of, Capoeta fusca, a cyprinid fish living in the qanats of south Khorasan, Iran: (Osteichthyes: Cyprinidae). Zoology in the Middle East 47(1), 63-70.‏
Kawser Ahmed M., Baki M.A., Kundu G.K., Islam S., Islam M., Hossain M. 2016. Human health risks from heavy metals in fish of Buriganga River, Bangladesh. SpringerPlus 5(1), 1-12.‏
Kharkan, J., Sayadi, M. H., Hajiani, M., Rezaei, M. R., & Savabieasfahani, M. 2022. Toxicity of nickel oxide nanoparticle in Capoeta fusca, using bioaccumulation, depuration, and histopathological changes. Global Journal of Environmental Science and Management 9(3), 1-18.
Kumar N., Gupta S.K., Bhushan S., Singh N.P. 2019. Impacts of acute toxicity of arsenic (III) alone and with high temperature on stress biomarkers, immunological status and cellular metabolism in fish. Aquatic Toxicology 214, 105233.‏
Liu F., Ni H.G., Chen F., Luo Z.X., Shen H., Liu L., Wu P. 2012. Metal accumulation in the tissues of grass carps (Ctenopharyngodon idellus) from fresh water around a copper mine in Southeast China. Environmental Monitoring and Assessment 184(7), 4289-4299.‏
Liu H., Fu S., Zhang S., Ding M., Wang A. 2020. Lead induces structural damage, microbiota dysbiosis and cell apoptosis in the intestine of juvenile bighead carp (Hypophthalmichthys nobilis). Aquaculture 528, 735573.‏
Mallatt J. 1985. Fish gill structural changes induced by toxicants and other irritants: a statistical review. Canadian Journal of Fisheries and Aquatic Sciences 42(4), 630-648.
Mansouri B., Ebrahimpour M., Babaei H., Farhangfar H. 2012. Bioaccumulation and elimination rate of cobalt by Capoeta fusca under controlled conditions. Journal of Animal & Plant Sciences, Lahore 22(3), 622-626.
Mansouri B., Maleki A., Davari B., Johari S.A., Shahmoradi B., Mohammadi E, Shahsavari S. 2016. Histopathological effects following short-term coexposure of Cyprinus carpio to nanoparticles of TiO 2 and CuO. Environmental Monitoring and Assessment 188(10), 575.
Mohamed F.A. 2009. Histopathological studies on Tilapia zillii and Solea vulgaris from Lake Qarun, Egypt. World Journal of Fish and Marine Sciences 1(1), 29-39.‏‏
Montaser M., Mahfouz M.E., El-Shazly S.A., Abdel-Rahman G.H., Bakry S. 2010. Toxicity of heavy metals on fish at Jeddah coast KSA: Metallothionein expression as a biomarker and histopathological study on liver and gills. World Journal of Fish and Marine Sciences 2(3), 174-185.‏
Mustafa S.A. 2020. Histopathology and heavy metal bioaccumulation in some tissues of Luciobarbus xanthopterus collected from Tigris River of Baghdad, Iraq. The Egyptian Journal of Aquatic Research 46(2), 123-129.‏
Naz S., Hussain R., Ullah Q., Chatha A.M.M., Shaheen A., Khan R.U. 2021. Toxic effect of some heavy metals on hematology and histopathology of major carp (Catla catla). Environmental Science and Pollution Research 28(6), 6533-6539.‏
Pariza A.A.F.M., Ahmad S.A., Fadzil N.I., Basirun A.A., Sha'arani S.A.W., Asri N.A.M., Shukor M.Y. 2019. Histopathological and cholinesterase changes in the gills of Clarias gariepinus as a result of cadmium exposure. Journal of Environmental Biology 40(4), 683-690.‏
Patel J.M., Bahadur A. 2010. Histopathological alternations in Catla catla induced by chronic exposure of copper ions. Journal of Cell and Tissue Research 10(3), 2365-2370.
Pirsaheb M., Azadi N.A., Miglietta M.L., Sayadi M.H., Blahova J., Fathi M., Mansouri B. 2019. Toxicological effects of transition metal-doped titanium dioxide nanoparticles on goldfish (Carassius auratus) and common carp (Cyprinus carpio). Chemosphere 215, 904-915.‏
Prabhu S., Poulose E.K. 2012. Silver nanoparticles: mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects. International Nano Letters 2(1), 32.
Purwanti I., Arroisi W., Rahardja B.S., Sulmartiwi L. 2019. Bioaccumulation and histopathological effect on the gills and liver of silver barb (Barbonymus gonionotus) exposed to the heavy metal nickel. In IOP Conference Series: Earth and Environmental Science 236(1), 012098.‏
Ribeiro C.O., Neto F.F., Mela M., Silva P.H., Randi M.A.F., Rabitto I.S., Pelletier E. 2006. Hematological findings in Neotropical fish Hoplias malabaricus exposed to subchronic and dietary doses of methylmercury, inorganic lead, and tributyltin chloride. Environmental Research 101(1), 74-80.
Saulea M., Stoica A.I., Baiulescu G.E., Marinescu D., Ionica, M. 2004. Determination of cobalt in food samples. Revista de Chimie 55(5), 301-303.‏
Sayadi M.H., Mansouri B., Shahri E., Tyler C.R., Shekari H., Kharkan J. 2020. Exposure effects of iron oxide nanoparticles and iron salts in blackfish (Capoeta fusca): Acute toxicity, bioaccumulation, depuration, and tissue histopathology. Chemosphere 247, 125900.‏
Sayadi M.H., Pavlaki M.D., Loureiro S., Martins R., Tyler C.R., Mansouri B., Shekari H. 2022. Co-exposure of zinc oxide nanoparticles and multi-layer graphenes in blackfish (Capoeta fusca): evaluation of lethal, behavioural, and histopathological effects. Ecotoxicology 31(3), 425-439.‏
Sayadi M.H., Pavlaki M.D., Martins R., Mansouri B., Tyler C.R., Kharkan J., Shekari H. 2021. Bioaccumulation and toxicokinetics of zinc oxide nanoparticles (ZnO NPs) co-exposed with graphene nanosheets (GNs) in the blackfish (Capoeta fusca). Chemosphere 269, 128689.‏
Sayadi, M.H., Kharkan, J. 2022. Investigating the toxicity potential of heavy metals on blood and biochemical indices of black fish. Journal of Natural Environment. In Press.
Taha M.M., Mahdy-Abdallah H., Shahy E.M., Ibrahim K.S., Elserougy S. 2018. Impact of occupational cadmium exposure on bone in sewage workers. International Journal of Occupational and Environmental Health 24(3-4), 101-108.‏
Tchounwon P.B., Yedjou C.G., Patlolla A.K., Sutton D.J. 2012. Heavy metal toxicity and the environment. Molecular, Clinical and Environmental Toxicology, Experentia Supplementum 101, 133-164.‏
Verkleji J.A.S. 1993. The effects of heavy metals stress on higher plants and their use as biomonitors. Plant as bioindicators: indicators of heavy metals in the terrestrial environment. VCH, New York. pp. 415-424.
Wong P.K. 1988. Mutagenicity of heavy metals. Bulletin of Environmental Contamination and Toxicology 40(4), 597-603.
Woody S.A., O'Neal S.L. 2015. Effects of Copper on Fish
and Aquatic Resources. The Nature Concervancy 27.
Yu Y., Tong B., Liu Y., Liu H., Yu H. 2021. Bioaccumulation, histopathological and apoptotic effects of waterborne cadmium in the intestine of crucian carp Carassius auratus gibelio. Aquaculture Reports 20, 100669.‏
Yuen B.B., Wong C.K., Woo N.Y.S., Au D.W. 2007. Induction and recovery of morphofunctional changes in the intestine of juvenile carnivorous fish (Epinephelus coioides) upon exposure to foodborne benzo [a] pyrene. Aquatic Toxicology 82(3), 181-194.