Culture of Freshwater Cladoceran Daphnia magna Using Scenedesmus, Animal Fertilizer, and Vegetative Powder

Document Type : Original Article

Authors

1 دانشکده منابع طبیعی، دانشگاه صنعتی اصفهان، اصفهان، ایران

2 Department of Natural Resources, Isfahan University of Technology, Isfahan, Iran

Abstract

In this study, effect green algae (Scenedesmus quadricauda), manure (cattle + poultry, 1:1 ratio in weight), vegetable (spinach+parsely+coriander,1:1:1 ratio in weight), manure+soil, vegetable+soil, and algae+soil were examined on culture of freshwater cladocerans, Daphnia magna. The experiment was carried out as completely randomized design with three replications under laboratory conditions. Results showed that the maximum population density (1513.9±627.1 individuals/L) (mean±SE) in algae treatment and maximum specific growth rate (SGR) (0.25±0.03 /day) and minimum doubling time (Dt) (3±0.7days) were obtained when D. magna fed on algae+soil. On day 30 of culture, the treatment of manure and manure+soil had highest adult population while the highest neonate percentage was observed at treatments of vegetable+soil, algae and algae+soil, respectively. The density, SGR, adult and neonate in population of D. magna significantly correlated with pH, BOD and COD. The maximum body length (2321.9 ± 149.3 µm) and width (1446.9 ± 66.8 µm) of D. magna measured when fed with manure. In conclusion, the treatments of algae and algae+soil had better performance based on growth and production as well as considerable decreasing in BOD and COD compared to other treatments.

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