This study was investigated organic (glucose, cholesterol, total protein and albumin) and ionic (calcium, phosphorus, magnesium, sodium and potassium) composition of the ovarian fluid and blood serum of 6 males (mean weight 424.37±37.84 g and mean length 36.19±1.32 cm) and 9 females (mean weight 1225.87±89.47 g and mean length 50.5±0.6 cm) of snow trout Schizothorax zarudnyi broods. Also, the sperm motility duration in combination of seminal fluid with 0, 15, 30, 45, 60, 75, 90 and 100% of ovarian fluid was measured. The ovarian fluid solution alone (100%) did not increase sperm motility. The shortest duration of sperm motility (44.67±0.56 s) with pure water and the highest duration of motility (67±5.37 s) was obtained by combining 55% water and 45% ovarian fluid that showed significant different with groups contain 0, 15, 30 and 60% of the ovarian fluid (P<0.05). There were differences in the composition of ovarian fluid, semen and blood serum. With the exception of magnesium, all other parameters of the female blood and ovarian fluid and all male blood and semen parameters were significantly different. Inaddition, there was a significant difference in the glucose, and total protein between blood serum of males and females. Comparison composition of the ovarian fluid, seminal fluid and blood serum of female and male broods showed the highest glucose and total protein in blood male serum, highest cholesterol and sodium in female and male blood serum, highest calcium in female blood serum, lowest magnesium and highest potassium in seminal fluid. The results showed increasing of the sperm motility duration with ovarian fluid at median concentrations in the activation medium. Also, the results revealed a possible important female contribution to sperm motility enhancement during the fertilization process in S. zarudnyi.