This experiment was conducted to evaluate some of the hematological (RBC, WBC, MCV, MCH, MCHC, Hb, PCV) and serum biochemical factors (TSP, GLU, COR, AST,ALT and ALP) of 2 to 3 year’s old Great Sturgeon during the yearlong period (autumn 2015 to summer 2016) at the Sturgeon Institute, Shahid Marjani in different seasons. For this purpose, after the seasonal blood sampling a total of 15 great sturgeon specimens were apparentlyhealthy after bleeding in the caudal vein hematological and serum biochemical indices was determined by standard protocols. Based on the results, seasonal variations were observed in WBCS, PCV, Hb, MCV, MCH, MCHC values, activity of AST, ALP enzymes and glucose and cortisol levels (P<0.05). But, there were no any significant differences between RBCs number’s, TSP levels and ALT values (P>0.05). Based on the results and according to the stable of all environmental conditions, except for temperature, couldbe claimed that temperature changes can be very important as a key factor in determining of hematological and biochemical indices of Huso huso in the different season that were complementary to the changes in water quality parameters indicating that environmental changes affects the blood chemistry of Huso huso. Overally, the hematological and biochemical values of the present study can be used as reference for monitoring of physiological and health status of Huso huso from Sturgeon Institute, Shahid Marjani and other center of the fishing and processing sturgeon fishes.