Aquaculture Sciences

Aquaculture Sciences

Bio-typing and characterization of whole cell and outer membrane proteins of Yersinia ruckeri isolates recovered from some rainbow trout (Onchorhynchus mykiss) farms in Iran

Document Type : Original Article

Authors
Ali Taheri Mirghaed 1
Sina Mirmazlumi 2
Mehdi Soltani 2
Pulin Shohreh 3
Ashkan Zargar 2
Elahe Soltani 4
1 Departmant of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
2 Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
3 Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran.
4 Department of microbiology, Faculty of Sciences, University of Tehran, Tehran, Iran.
Abstract
Yersinia ruckeri is the etiological agent of Yersinia septicemia or yersiniosis of farmed fishes. In this study, Y. ruckeri isolates were isolated and characterized in the case of phenotype, biotype as well as their patterns of whole cell and outer membrane proteins. Seven isolates were recovered from rainbow trout farms in the Mazandaran, Chaharmahal-va-Bakhtiari, Kohgiluyeh-va-Boyerahamad, and Zanjan provinces during spring and summer 2018.  These isolated Yersinia plus five previously recovered isolates form the North Khorasan and Western Azarbaijan Gharbi were identified as Y. ruckeri based on their phenotype, biotype and molecular characterization. All isolates were biotype one with the high identical patterns of whole cell proteins and outer membrane proteins in electrophoresis. These results illustrated that Y. ruckeri as the cause of yersiniosis in farmed rainbow trout in Iran Y. ruckeri isolates are mostly homogenous showing low diversity and thus, could be useful in further preventive studies such as vaccination.
Keywords
Yersinia ruckeri
Whole cell protein
Outer membrane protein
Bio type