Comparative study of different levels of dietary oligofructose and inactive yeast on intestinal microbiota of beluga sturgeon Huso huso (Linnaeus, 1758)

Document Type : Original Article

Author

Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

Abstract

The aim of this study was comparative study of the effects of two prebiotics, oligofructose and inactive yeast, on intestinal microbiota of beluga (Huso huso). The study was done in a completely randomize design include feeding beluga with diet contain 1 or 2% inactive yeast and 1 or 2% oligofructose as well as a control group (5 treatments in triplicates). Beluga (11.40±0.56 g) supplied, 35 specimens stocked in each fiberglass tanks and were fed for 6 weeks with experimental diets. At the end of trial total viable lactic acid bacteria (LAB) and their proportion was studied using culture based method. Our results showed that administration of oligofructose or inactive yeast had no significant effects on total viable bacteria compared control group (P > 0.05). The highest LAB levels was observed in 2% oligofructose or inactive yeast fed fish which was significantly higher than control (P < 0.05). The proportion of LAB to total viable bacteria was significantly elevated in all prebiotic fed fish (P > 0.05) and the highest proportion observed in 2% oligofructose fed beluga.

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