Effect of diluent solutions on the fertilization of snow trout (Schizothorax zarudnyi)

In this study, the effects of semen diluent solutions on improvement of reproduction performance, by using the criteria such as: the rates of fertilization, percentage of eyed egg and hatching rates were studied. For this purpose eight female broods with average weight and length 1545.62±295.43 g and 54.37±3.85 cm, respectively and sixteen male broods were selected with average weight and length 623.08±180.69 g diluent solutions and 38.91±5.40 cm, respectively. After separation of eggs from coelomic fluids, they were fertilized with sperm using four different diluent solutions: 1- 5.54 g NaCl, 2.422 g Triss, 3.57 g Glycin, regulated in pH=8.4 and solution in 1L distilled water. 2- 7.305 g NaCl, 0.735 g CaCl₂.2H₂O, regulated in pH=8.4 and solution in 1L distilled water. 3- coelumic fluid that was separated from egg by using net and 4- distilled water as control treatment. The results showed that motility duration of sperm in 1^th, 2^nd, 3^ed and 4^th treatments was 65.25±7.36, 30.8±12.83, 73.33±2.117, 40.00±1.61 sec, the duration of forward movement was 6.25±4.71, 15.00±12.63, 52.33±1.90, 34.66±2.51 sec. and percentage eyed eggs was 16.82±6.42, 53.00±4.66, 82.50±3.84, 73.33±2.08, respectively. Percentage hatching was 55.05±5.42, 71.43±0.81, 49.60±6.25, 37.72±4.32, respectively. The results of spermatology parameters, eyed egg percentage and hatching rates showed a significant differences between the 2^nd treatment with the rest (P≤0/05). Thus, it can be suggested the 2^nd treatment is an effective diluents solution for improving breeding performance of S. zarudnyi.