Comparative study of efficiency of prebiotic with different degree of polymerization on intestinal microbiota of Carp (Cyprinus carpio) larvae

Evaluation of different prebiotics showed contradictory results on intestinal microbiota which was mainly depends on the degree of polymerization. Therefore, the present study was performed to investigate the effects of different levels of two prebiotics, Inulin and oligofructose with different degree of polymerization on intestinal microbiota of Carp (Cyprinus carpio) larvae. The study was performed as randomized design with 5 treatments and 3 replications in which carp larvae were fed different levels, 0, 0.5 and 1% of Inulin and oligofructose with different 23 and 5 degree of polymerization, respectively (Purity 92%) for 8 weeks. At the end of the feeding trial culture based analysis of intestinal microbiota include lactic acid bacteria levels count agar media. Administration of different levels of Inulin and oligofructose had no significant effects on total bacteria of intestinal microbiota (P > 0.05). The lactic acid bacteria levels were significantly increased compared following prebiotics administration in diet (P > 0.05). LAB levels in intestinal microbiota of oligofructose fed fish were significantly higher than those in Inulin group. The highest LAB proportion in intestinal microbiota was observed in carp fed diet contains 1% oligofructose (P > 0.05). The results of the present study revealed that prebiotics can be used for modulation of carp intestinal microbiota toward beneficial bacterial communities. Furthermore, administrations of the prebiotic with lower degree of polymerization are more efficient for modulation of intestinal microbiota and elevation of LAB levels.