Effect of brood fish age on first cleavage interval to improve the efficiency of tetraploid induction in rainbow trout (Oncorhynchus mykiss)

Document Type : Original Article

Authors

1 Department of Natural Resources, Isfahan University of Technology, Isfahan, 84156-83111, Iran.

2 Department of Agriculture, Isfahan University of Technology, Isfahan, 84156-83111, Iran.

3 Department of Biology, University of New Brunswick, Fredericton, New Brunswick, E3B 6E, Canada.

Abstract

First cleavage interval (FCI) determination in fertilized fish eggs has a key role in improving late shock for tetraploid induction. This study was conducted to investigate the effect of female brood fish age 2+ and 4+ years on FCI to optimize the time of shock induction to obtain the highest tetraploidy. 22 and 16 female brood fish at 2+ and 4+ year class were selected with an average weight 1635±357.83 and 4242.5±1039.36 g, respectively. The female fish were hand stripped and the eggs from each breeder were separately fertilized by the mix of 3 male milt. The eggs then were incubated separately in 3 replicates for each female. The time of adding the activating solution was recorded as the fertilization time. Sampling was started at 4620 degree-min after fertilization (at 11°C) and continued at 10-min intervals for 1980 degree-min. At least, 30 of eggs from each female fish, 10 from each replicate from each female were randomly collected at each sampling time. The fish eggs were fixed and then checked at 40x magnification. FCI in at least 50% of sampled eggs was considered as the time of occurrence of FCI for the female fish. The results showed that FCI occured in eggs from younger breeders significantly in a shorter time after fertilization compared to the older group. The average FCI was recorded as 5528.64±104.06 and 5802.5±60.16 degree-min for 2+ and 4+ respectively. A low regression was recorded between FCI and egg size. So, it is recommended to consider the female fish age for optimizing tetraploidy yield using late shock in rainbow trout.

Keywords

References

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Aquaculture Sciences
Volume 11, Issue 1
March 2023
Pages 142-151

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