In this study, the effects of Nano emulsions of clove oil (NCL), nano emulsions of lemonbeebrush plus clove oil (NLBC) and Tricaine Methanesulfonate (MS222) as anesthetic agents were investigated on blood parameters for rainbow trout (Oncorhynchus mykiss). The experiments were accomplished by 120 fish with average weight 50±12 g. The fish were sorted into 3 treatments each with 3 replicates (consisting of 10 fish in each replicate. The treatments consisted of NBLC with 10 mg/l concentration (T1) and NCL with 10 mg/l concentration (T2) and MS with 10 mg/l (T3). Bath method was used for anesthetic bath treatments. The control group was not exposed to any type of the anesthetic. The anesthetics treatments and control were saturated with oxygen. The time to reach the sedation stage was recorded. Fish were kept in sedation stage for 24h and then the chemical parameters of water were measured. 3 fihses randomly were selected from treatments and blood samples were collected through caudal vein. Blood biochemical (Urea, Na, Cl, K) and immunity parameters including (lactate, glucose, cortisol, lysozyme, SOD, ACH50) were measured. The results showd that NBLC can significantly decrease oxygen consuption and ammonia excretion compared to the control (P<0.05). The results of non-specific immune parameters showed that NLBC could significantly improve the glucose, lysozyme and superoxide dismutase (SOD). The result of this experiment clearly indicated that, NBLC induced sedation quicker comparing to other treatments, without any mortality. However, the result of this experiment indicated that, NBLC have the most effect comparing to NCL and MS222.