The effect Malva neglecta on growth, survival and heamatological parameters in Cyprinus carpio

Document Type : Original Article

Authors

Department of Aquaculture, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran.

Abstract

This survey was done to study the effect of the powder of Malva neglecta as a natural immune stimulant on growth factors and heamatological in Cyprinus carpio in summer 2016 during 60 days rearing period in aquaculture center of Islamic azad university, Ahvaz Branch. After adaptation, a totalof 360 fish were placed in 12 300-liters tanks, that 30 of fish (initial weight, 18.10±0.18 gr) were in every tank. The treatments were included control (0 gr/Kg), treatment 1 (2%), treatment 2 (4 %) and treatment 3 (6%) of Malva neglecta powder. The results of defence cells in Cyprinus carpio were showed that Monocyts and Basiphyls observed just in treatment 1 and 2. The highest Lymphocyts and  Noutrophilsw were  counted respectively 95.5% (treatment 3) 48.16% (treatment 2). The Eosinophyls were not present in any of treatments. MCH (1.12 µm310-1) was showed in treatment three. The  highest of complement factor and killing bacterial serum power were caculated in treatment 2 comparison to control (P<0.05) but the highest of Lysosim observed in treatment 3 (55.54 g/dI) (P<0.05). The highest MCV, MCHC, WBC, RBC, Hg and HCT in treatment 3 were calculated respectively 1.12 (µm3)10-1, 5.59 (µm3)10-1, 3.37 (µm3)10-1, 173.33 (103cell.mm-3(, 1.36 )106 cell.mm-3 (8.3 (g/dI) and 40.66%. The Malva neglecta powder were showed no significant difference in growth factor and survival in Cyprinus carpio (P>0.05) but were observed 100% survival in fish in treatment 1, 2 and 3. The Value of 4% of powder Malva neglecta in diet caused improvement heamatological factors in Cyprinus carpio. Also 2 percent of powder in diet were cause of increasing complement factor and killing bacterial  serum in this fish.
 

Keywords