نوع مقاله : مقاله پژوهشی
گروه تکثیر و پرورش آبزیان، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.
عنوان مقاله [English]
The present study investigates the effect of dietary administration of the hydroalcoholic extract of fennel (Foeniculum vulgare) on expression of genes related to reproduction (ER-a and Vtg) in female zebrafish (Danio rerio). For this purpose, zebrafish with average wight of 0.15±0.01 gr fed on diet supplemented with 0, 75, 100 and 150 mg/kg of diet for 6 weeks. After this period and sex determination, females fed with fennel for 1 month. At the end of feeding trial, RNA extracted from liver, cDNA syntesised with SuPrimeScript RTase kit and PCR were done using primers relate to Vtg, ER-a and Beta-actin as housekeeping genes. Evaluation of reproduction gene expression showed increase in fish fed fennel compare to control. Vtg and ER-a gene expression didn’t show significant differences in fish fed 75and 100 mg/kg (P>0.05). Diet supplemented with 150 mg/kg of fennel showed significant increases compared to two other fennel-fed groups. According to the results it seems that using fennel could considered as a new strategy for improving reproductive performance.