تأثیر ریزپلاستیک‌ها با رنگ‌های مختلف بر برخی از شاخص‌های استرس اکسیداتیو در ماهی Gambusia holbrooki Girard, 1859

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه شیلات، دانشکده منابع طبیعی دانشگاه صنعتی خاتم الانبیا (ص) بهبهان، اﯾﺮان.

چکیده

هدف از انجام این آزمایش ارزیابی تاثیر ریزپلاستیک­ های رنگی بر شاخص­ های استرس اکسیداتیو در ماهی گامبوزیا (Gambusia holbrooki) بوده است. فرضیه این مطالعه این بود که ریسک بروز استرس اکسیداتیو در ماهیان ممکن است براساس رنگ ریزپلاستیک ­ها متفاوت باشد. ماهیان در 7 تیمار آزمایشی شامل گروه کنترل و 6 گروه آزمایشی در معرض 150 میکروگرم در لیتر ریزپلاستیک­ های رنگی شامل رنگ­ های سفید، سبز، زرد، قرمز، آبی و سیاه برای 14 روز قرار گرفتند. در پایان قابلیت تجمع زیستی ریزپلاستیک­ ها با دستگاه FTIR و برخی از شاخص­ های استرس اکسیداتیو اندازه­ گیری شد. نتایج نشان داد که فعالیت سوپراکسید دیسموتاز در کبد ماهیان در معرض ریزپلاستیک ­های سیاه، زرد و قرمز به‌طور معنی ­داری  بیشتر از گروه کنترل است (P<0.05). علاوه بر این، قرار گرفتن ماهیان در معرض ریزپلاستیک­ های سیاه، سبز و قرمز منجر به افزایش معنی ­دار در فعالیت آنزیم کاتالاز، گلوتاتیون­پراکسیداز و گلوتاتیون­رودکتاز در سلول­های کبدی شد(P<0.05). این نتایج نشان می­ دهد که احتمال بروز آسیب­ های اکسیداتیو در ماهیان در معرض ریزپلاستیک ­های سیاه، سبز و قرمز به‌طور معنی ­داری بیشتر از دیگر ریزپلاستیک ­های رنگی بوده است.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of microplastics with different colors on some oxidative stress biomarkers in Gambusia holbrooki Girard, 1859

نویسندگان [English]

  • Mehdi Banaee
  • Marzieh Mohammadzadeh
Department of Aquaculture, Faculty of Natural Resources, Khatam Al-Anbia University of Technology, Behbehan, Iran.
چکیده [English]

This experiment aims to evaluate the effect of colored microplastics (MPs) on oxidative stress indicators in Gambusia holbrooki. This study hypothesized that the risk of oxidative stress in fish may be different based on MPs’ color. In this study, fish in 7 experimental treatments with two replications, including the control group and six experimental groups, were exposed to 150 micrograms per liter of colored MPs (white, green, yellow, red, blue, and black colors) for 14 days. In the end, some oxidative stress indicators were assayed. Furthermore, the bioaccumulation potential of MPs was measured with an FTIR instrument. The results showed that the activity of superoxide dismutase in the liver of fish exposed to black, yellow, and red MPs is significantly higher than in the control group (P<0.05). In addition, exposure of fish to black, green, and red MPs led to a significant increase in the activity of catalase, glutathione-peroxidase, and glutathione-reductase in liver cells (P<0.05). These results showed that the risk of oxidative stress in fish exposed to black, green, and red MPs was significantly higher than other colored MPs.

کلیدواژه‌ها [English]

  • Colored microplastics
  • Gambusia
  • FTIR
  • Oxidative stress indicators
جعفری­کناری س­س.، ادهمی ب. 1394. مقایسه تغذیه­ای جیره بیومار، میگو، کرم خونی بر پارامترهای رشد، بازماندگی و فراسنجه­های خونی در بچه ماهی آکواریومی جوئل (Hemichromis bimaculatus). علوم تکثیر و آبزی­پروری. 3(7): 52-43.
محمدنژادشموشکی م،. حیدری س.، موسوی­ثابت س­.ح. 1390. مقایسه تغذیه­ای جیره بیومار، دل گوساله، کرم فشرده، کرم خونی، گاماروس و آرتمیا بر روی شاخص­های رشد و بازماندگی ماهی سوروم (Heros severus). زیست­شناسی جانوری. 3(3): 49-41.
Banaee M., Gholamhosseini A., Sureda A., Soltanian S., Fereidouni, M.S., Ibrahim, A.T. 2021. Effects of microplastic exposure on the blood biochemical parameters in the pond turtle (Emys orbicularis). Environmental Science and Pollution Research 28(8), 9221-9234.
Banaee M., Shakeri, R. 2021. The effect of microplastics on hematological and biochemical parameters in aquatic animals. Journal of Aquaculture Sciences 8(15), 141-160.
Banaee M., Soltanian S., Sureda A., Gholamhosseini A., Haghi B N., Akhlaghi M., Derikvandy A. 2019. Evaluation of single and combined effects of cadmium and micro-plastic particles on biochemical and immunological parameters of common carp (Cyprinus carpio). Chemosphere 236, 124335.
Banihashemi E A., Soltanian S., Gholamhosseini A., Banaee M. 2022. Effect of microplastics on yersinia ruckeri infection in rainbow trout (Oncorhynchus mykiss). Environmental Science and Pollution Research 29(8), 11939-11950.
Barboza L.G., Guilhermino L. 2018. Microplastics cause neurotoxicity, oxidative damage and energy-related changes and interact with the bioaccumulation of mercury in the European seabass, Dicentrarchus labrax (linnaeus, 1758). Aquatic Toxicology 195, 49-57.
Bebianno M.J., Mendes V.M., O'Donovan S., Carteny C.C., Keiter S., Manadas B. 2022. Effects of microplastics alone and with adsorbed benzo (a) pyrene on the gills proteome of Scrobicularia plana. Science of the Total Environment 842, 156895.
Bobori D.C., Dimitriadi A., Feidantsis K., Samiotaki A., Fafouti D., Sampsonidis I., Kalogiannis S., Kastrinaki G., Lambropoulo D.A., Kyzas G.Z., Koumoundouros G., Bikiaris D.N., Kaloyianni M. 2022. Differentiation in the expression of toxic effects of polyethylene-microplastics on two freshwater fish species: Size matters. Science of the Total Environment 830, 154603.
Bringer A., Cachot J., Dubillot E., Prunier G., Huet V., Clérandeau C., Evin L., Thomas H. 2022. Intergenerational effects of environmentally-aged microplastics on the Crassostrea gigas. Environmental Pollution 294, 118600.
Buwono N.R., Risjani Y., Soegianto A. 2022. Oxidative stress responses of microplastic-contaminated Gambusia affinis obtained from the Brantas River in East Java, Indonesia. Chemosphere 293, 133543.
Capó X., Company J. J., Alomar C., Compa M., Sureda A., Grau A., Hansjosten B., Lopez-Vazque J., Quintana J. B., Rodil R., Deudero S. 2021. Long-term exposure to virgin and seawater exposed microplastic enriched-diet causes liver oxidative stress and inflammation in gilthead seabream Sparus aurata, Linnaeus 1758. Science of the Total Environment 767, 144976.
Capo X., Rubio M., Solomando A., Alomar C., Compa M., Sureda A., Deudero S. 2021. Microplastic intake and enzymatic responses in Mytilus galloprovincialis reared at the vicinities of an aquaculture station. Chemosphere 280, 130575.
Critchell K., Hoogenboom M.O. 2018. Effects of microplastic exposure on the body condition and behaviour of planktivorous reef fish (Acanthochromis polyacanthus). PLoS One 13(3), e0193308.
Cunha C., Lopes J., Paulo J., Faria M., Kaufmann M., Nogueira N., Ferreira A., Cordeiro N. 2020. The effect of microplastics pollution in microalgal biomass production: A biochemical study. Water Research 186, 116370.
de Sá L C., Luís L. G., Guilhermino L. 2015. Effects of microplastics on juveniles of the common goby (Pomatoschistus microps): Confusion with prey, reduction of the predatory performance and efficiency, and possible influence of developmental conditions. Environmental Pollution 196, 359-362.
Ding J., Huang Y., Liu S., Zhang S., Zou H., Wang Z., Zhu W., Geng J. 2020. Toxicological effects of nano- and micro-polystyrene plastics on red tilapia: Are larger plastic particles more harmless? Journal of Hazardous Materials 396, 122693.
Ding J., Zhang S., Razanajatovo M.R., Zou H., Zhu W. 2018. Accumulation, tissue distribution, and biochemical effects of polystyrene microplastics in the freshwater fish red tilapia (Oreochromis niloticus). Environmental Pollution 238, 1-9.
Dong R., Zhou C., Wang S., Yan Y., Jiang Q. 2022. Probiotics ameliorate polyethylene microplastics-induced liver injury by inhibition of oxidative stress in Nile tilapia (Oreochromis niloticus). Fish and Shellfish Immunology 130, 261-272.
Esterhuizen M., Buchenhorst L., Kim Y.J., Pflugmacher S. 2022. In vivo oxidative stress responses of the freshwater basket clam Corbicula javanicus to microplastic fibres and particles. Chemosphere 296, 134037.
Félix L., Carreira P., Peixoto F. 2022. Effects of chronic exposure of naturally weathered microplastics on oxidative stress level, behaviour, and mitochondrial function of adult zebrafish (Danio rerio). Chemosphere 310, 136895.
Góth L. 1991. A Simple Method for Determination of Serum Catalase Activity and Revision of Reference Range. Clinica Chimica Acta 196, 143-152.
Hamed M., Soliman H. A., Badrey A. E., Osman A. G. 2021. Microplastics induced histopathological lesions in some tissues of tilapia (Oreochromis niloticus) early juveniles. Tissue and Cell 71, 101512.
Han M., Gao T., Liu G., Zhu C., Zhang T., Sun M., Li J., Ji F., Si Q., Jiang Q. 2022. The effect of a polystyrene nanoplastic on the intestinal microbes and oxidative stress defense of the freshwater crayfish, Procambarus clarkii. Science of the Total Environment 833, 155722.
Hu M., Palić D. 2020. Micro-and nano-plastics activation of oxidative and inflammatory adverse outcome pathways. Redox Biology 37, 101620.
Huang J.N., Wen B., Zhu J.G., Zhang Y.S., Gao J.Z., Chen Z.Z. 2020. Exposure to microplastics impairs digestive performance, stimulates immune response and induces microbiota dysbiosis in the gut of juvenile guppy (Poecilia reticulata). Science of the Total Environment 733:138929. 
Iheanacho S. C., Igberi C., Amadi-Eke A., Chinonyerem D., Iheanacho A., Avwemoya F. 2020. Biomarkers of neurotoxicity, oxidative stress, hepatotoxicity and lipid peroxidation in Clarias gariepinus exposed to melamine and polyvinyl chloride. Biomarkers 25(7), 603-610.
Kim J.H., Yu Y.B., Choi J.H. 2021. Toxic effects on bioaccumulation, hematological parameters, oxidative stress, immune responses and neurotoxicity in fish exposed to microplastics: A review. Journal of Hazardous Materials 413, 125423.
Lombardo J., Solomando A., Cohen-Sánchez A., Pinya S., Tejada S., Ferriol P., Mateu-Vicens G., Box A., Faggio C., Sureda A. 2022. Effects of Human Activity on Markers of Oxidative Stress in the Intestine of Holothuria tubulosa, with Special Reference to the Presence of Microplastics. International Journal of Molecular Sciences 23(16), 9018.
Lu Y., Zhang Y., Deng Y., Jiang W., Zhao Y., Geng J., Ding L., Ren H. 2016. Uptake and accumulation of polystyrene microplastics in zebrafish (Danio rerio) and toxic effects in liver. Environmental Science and Technology 50(7), 4054-4060.
Nematdoost Haghi B., Banaee M. 2017. Effects of micro-plastic particles on paraquat toxicity to common carp (Cyprinus carpio): Biochemical changes. International Journal of Environmental Science and Technology 14(3), 521-530.
Ory N. C., Gallardo C., Lenz M., Thiel M. 2018. Capture, swallowing, and egestion of microplastics by a planktivorous juvenile fish. Environmental Pollution 240, 566-573.
Pannetier P., Morin B., Bihanic F.L., Dubreil L., Clérandeau C., Chouvellon F., Arkel K.V., Danion M., Cachot J. 2020. Environmental samples of microplastics induce significant toxic effects in fish larvae. Environment International 134, 105047.
Pisani X. G., Lompré J. S., Pires A., Greco L. L. 2022. Plastics in scene: A review of the effect of plastics in aquatic crustaceans. Environmental Research 212, 113484.
Provenza F., Rampih D., Pignattelli S., Pastorino P., Barceló D., Prearo M., Specchiulli A., Renzi M. 2022. Mussel watch program for microplastics in the Mediterranean sea: Identification of biomarkers of exposure using Mytilus galloprovincialis. Ecological Indicators 142, 109212.
Song J A., Choi C. Y., Park H. S. 2020. Exposure of bay scallop Argopecten irradians to micro-polystyrene: bioaccumulation and toxicity. Comparative Biochemistry and Physiology Part C  236, 108801.
Sun T., Zhan J., Li F., Ji C., Wu H. 2021. Evidence-based meta-analysis of the genotoxicity induced by microplastics in aquatic organisms at environmentally relevant concentrations. Science of the Total Environment 783, 147076.
Umamaheswari S., Priyadarshinee S., Kadirvelu K., Ramesh M. 2021. Polystyrene microplastics induce apoptosis via ROS-mediated p53 signaling pathway in zebrafish. Chemico-Biological Interactions 345, 109550.
Uurasjärvi E., Sainio E., Setälä O., Lehtiniemi M., Koistinen A. 2021. Validation of an imaging FTIR spectroscopic method for analyzing microplastics ingestion by Finnish lake fish (Perca fluviatilis and Coregonus albula). Environmental Pollution 288, 117780.
Varó I., Perini A., Torreblanca A., Garcia Y., Bergami E., Vannuccini M. L., Corsi I. 2019. Time-dependent effects of polystyrene nanoparticles in brine shrimp Artemia franciscana at physiological, biochemical and molecular levels. Science of the Total Environment 675, 570-580.
Weinstein J.E., Ertel B.M., Gray, A.D. 2022. Accumulation and depuration of microplastic fibers, fragments, and tire particles in the eastern oyster, Crassostrea virginica: A toxicokinetic approach. Environmental Pollution 308, 119681.
Wen B., Jin S., Chen Z., Gao J., Liu Y., Liu J., Feng X. 2018. Single and combined effects of microplastics and cadmium on the cadmium accumulation, antioxidant defense and innate immunity of the discus fish (Symphysodon aequifasciatus). Environmental Pollution 243, 462-471.
Xiong X., Tu Y., Chen X., Jiang X., Shi H., Wu C., Elser J J. 2019. Ingestion and egestion of polyethylene microplastics by goldfish (Carassius auratus): influence of color and morphological features. Heliyon 5(12), e03063.
Zhang C., Wang J., Zhou A., Ye Q., Feng Y., Wang Z., Wang S., Xu G., Zou J. 2021. Species-specific effect of microplastics on fish embryos and observation of toxicity kinetics in larvae. Journal of Hazardous Materials 403, 123948.
Zhang X., Jin Z., Shen M., Chang Z., Yu G., Wang L., Xia X. 2022. Accumulation of polyethylene microplastics induces oxidative stress, microbiome dysbiosis and immunoregulation in crayfish. Fish and Shellfish Immunology 125, 276-284.
Zhao T., Tan L., Han X., Wang X., Zhang Y., Ma X., Lin K., Wang R., Ni Z., Wang J., Wang J. 2022. Microplastic-induced apoptosis and metabolism responses in marine Dinoflagellate, Karenia mikimotoi. Science of the Total Environment 804, 150252.