نوع مقاله : مقاله پژوهشی
1 گروه شیلات، دانشگاه هرمزگان، بندرعباس، ایران.
2 گروه شیلات، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.
عنوان مقاله [English]
This study was designed to evaluate the haemolymph immunological and antioxidant parameters in Penaeus vannamei fed on Sargassum ilicifolium hot-water extract (SHWE) supplemented diets. Three experimental groups including 0.25, 0.5, and 1% SHWE, and one control (without SHWE inclusion), each with three replicates, were applied. A totsl of 120 shrimps (4.67±0.08 g) were randomly distributed into 12 experimental tanks. At the end of a 66-day feeding trial, haemolymph immunological and antioxidant parameters were measured. The results indicated that alkaline phosphatase increased significantly in 0.25% SHWE fed treatment (p < /em><0.05). Glutathione peroxidase significantly increased in all the treatments fed the SHWE supplemented diets (p < /em><0.05). However, the levels of glutathione significantly increased in the groups fed by 0.25 and 0.5% SHWE supplemented diets (p < /em><0.05). The levels of glutathione s-transferase, superoxide dismutase, and catalase significantly increased in the treatment fed 0.5% SHWE supplemented diet (p < /em><0.05), whereas the total antioxidant capacity levels remained unchanged among the treatments (p < /em>>0.05). Both acid phosphatase and phenoloxidase did not influenced by dietary treatments (p < /em>>0.05). The levels of lysozyme were significantly lower in the treatment fed 0.25% SHWE supplementary (p < /em><0.05), whereas this experimental group did not show any difference with the others (p < /em>>0.05). The numbers of THC, GC, SGC, and HC significantly increased in the treatment fed 0.5% SHWE supplemented diet (p < /em><0.05). However, the fish fed 0.25% SHWE supplemented diet exhibited significantly higher THC and SGC levels, compared to the control group (P>0.05). The present results indicate that SHWE can be applied practically via a dietary route to boost the haemolymph antioxidant status as well as the cellular immunity of Pacific white shrimp.