Aquaculture Sciences

Aquaculture Sciences

The efficacy of HOCL-based solution (Sanitizon) against microbial load in Rainbow trout farms

Document Type : Original Article

Authors
Golnaz Sayyadzadeh
Somaieh Sabzali
Department of Biology, Faculty of Sciences, Lorestan University, Khorramabad, Iran.
Abstract
Simultaneous control of pathogenic bacteria and fungi in aquaculture is a major challenge. This study investigated the antimicrobial effects of hypochlorous acid (HOCl) disinfectant on bacterial and fungal populations in water and evaluated its safety for fish. The experiment was conducted on a rainbow trout farm. The treatments were applied at three concentrations (50, 100, and 150 ppm) of HOCl solution. Water samples were collected before and 2, 5, and 10 min after treatment, and the total number of viable bacteria and fungi was calculated. Fish behavior and mortality were monitored for 24 hours. HOCl concentrations of 50 and 100 ppm led to a decrease in the number of bacteria and fungi over a period of 2, 5 and 10 minutes. At these two concentrations, a significant reduction in bacteria population was observed but complete removal was not performed. These reductions were significant at each studied time and dilution compared to the previous time and dilution. Concentration of 150 ppm at all times was a severe decrease and nearly zero of the number of fungal and bacterial colonies identified from the studied water. The results of this study indicate that a commercially available sanitizon solution can be a rapid and effective broad-spectrum disinfectant against bacteria and fungi in fish pond water. This substance has low toxicity to fish, making it a safe and practical option for use in aquaculture.
Keywords
Hypochlorous acid
Aquaculture
Fish health
Disinfection
Sanitizon

Subjects

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Aquaculture Sciences
Volume 13, Issue 2
October 2025
Pages 100-109