Optimization of the extraction of antioxidant compounds from brown algae Sargassum angustifolium in the Persian Gulf by response surface methodology (RSM)

Document Type : Original Article

Authors

1 Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Guilan, Iran.

2 Department of Fisheries, Faculty of natural Resources and Marine Sciences, Tarbiat Modares University, Noor, Iran.

Abstract

In this study, optimum conditions for the antioxidant compounds extraction from Sargassum angustifoliumalgae were determined by response surface methodology. Optimal design with three independent variables: ethanol concentration (with five levels of 0, 25, 50, 75 and 100), extraction time (with three levels of 2, 4 and 6 hours) and solid-liquid ratio (with three levels of 1: 5, 1:10 and 1:15) was generated. Total phenolic content (TPC), free radical inhibitory activity (DPPH) and total antioxidant capacity (TAC) were used as responses. The results showed that the maximum TPC in optimal conditions was obtained in distilled water, 6 hours and a 1: 15 solid-liquid ratio, free radical inhibitory activity in ethanol 25%, 4 hours duration, and a solid-liquid ratio of 1: 5 and total antioxidant capacity in ethanol 50%, duration 4 hours and solid-liquid ratio 1:15. In terms of the above mentioned conditions, the highest amount of TPC was 698.01 mg gallic acid per gram of dried algae powder, TAC value was 76.1 mg ascorbic acid per gram of dried algae powder and DPPH value was 86.02 (RSA%). The results showed that the extract of S. angustifolium could be used as a natural antioxidant in the food industry, as well as food supplementation against oxidative damage.

Keywords

Aquaculture Sciences
Volume 8, Issue 1 - Serial Number 1
September 2020
Pages 43-58

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